Study testing a new experimental cell therapy in adults and children With Relapsed or Refractory Acute Myeloid Leukemia and Blastic Plasmacytoid Dendritic Cell Neoplasm

Prior treatment

• Evidence of significant, uncontrolled concomitant diseases that could affect compliance with the protocol or interpretation of results, including diabetes mellitus, history of relevant pulmonary disorders (bronchospasm, obstructive pulmonary disease) and known autoimmune diseases
• Treatment with any other standard anti-cancer radiotherapy/chemotherapy (except hydroxyurea,) iwithin 2 weeks prior to Dual CARCIK infusion
• Treatment with investigational therapy (defined as treatment for which there is currently no regulatory authority approved indication) 14 days prior to lymphodepletion or five half-life duration of the drug, or whichever comes first
• Toxicities from prior anti-cancer therapy including immunotherapy that did not resolve to ≤ Grade 1 with the exception of alopecia, endocrinopathy managed with replacement therapy and stable vitiligo
• Prior treatment with CAR-T cell therapy within 30 days before first study treatment administration (lymphodepletion)
• Patients with a prior history of Grade 4 or higher for CRS and ICANS after CAR-T cell therapy
• Patients with a prior history of Grade 4 or higher toxicity following anti-CD33– or anti-CD123 targeted therapy
• Active GvHD Grades II-IV or moderate/severe chronic GvHD requiring systemic immunosuppressive therapy (for patients who had previously been allotransplanted)
• Administration of a live, attenuated vaccine within 4 weeks prior to CARCIK-CD123/33 infusion.
• Presence of clinically meaningful donor specific anti-HLA antibodies (DSA) against CARCIK-CD123/33 donor haplotype HLA typing (only in case of HLA mismatched)
• Class III/IV cardiovascular disability according to New York Heart Association (NYHA) classification, myocardial infarction or cerebrovascular accident within 30 days prior to Dual CARCIK infusion, unstable arrhythmias, or unstable angina)

Other illness

• Patients with clinically significant active viral, bacterial or fungal infection
• Patients with an active infection with Hepatitis B
• Patients with an active hepatitis C virus (HCV) infection. However, patients with successfully treated chronic HCV infection will be allowed if they show a sustained virologic response at 12 weeks (SVR12) or 24 weeks (SVR24), and if there is a 4-week period between achieving sustained viral response (SVR12 or SVR24) and time of informed consent.
• HIV Positive test (serology or PCR)
• Rapidly progressive disease that in the estimation of the investigator and sponsor could affect compliance with the protocol or interpretation of results
• Patients with another active invasive malignancy. Only patients with completely resected carcinoma in situ not requiring treatment other than surgery can be considered for treatment.

Lab values

• Allogeneic or autologous HCT within 3 months or Donor Lymphocytes (DLI) within 1 month prior to CARCIK-CD123/33 infusion.

Other

• Uncontrollable CNS leukemia or neurological symptoms defined as CNS grade 3. A CNS evaluation should be performed as clinically appropriate to rule out CNS involvement.

• Patients/parents or legal representatives able and willing to provide written informed consent and to comply with the study protocol according to ICH and local regulations. Age-appropriate assent from children will also be requested.
• Life expectancy (in the opinion of the Investigator) of > 12 weeks
• Females of childbearing potential (FCBP) subjects must: a) Have a negative pregnancy test as verified by the local investigator b) Either commit to true abstinence from heterosexual contact or agree to use, and be able to comply with, effective contraception without interruption from screening until at least 12 months following CARCIKCD123/33 infusion and until CAR positive viable T cells are no longer present by quantitative polymerase chain reaction (qPCR) on two consecutive tests. c) Contraception methods must include one highly effective method, like: surgical female sterilization, use of oral (estrogen and progesterone), injected, or implanted hormonal methods of contraception or placement of an intrauterine device (IUD) or intrauterine system (IUS), or other forms of hormonal contraception that have comparable efficacy (failure rate <1%) d) Agree to abstain from breastfeeding during study participation and for at least 12 months following V infusion and until CAR positive viable T cells are no longer present by quantitative polymerase chain reaction (qPCR) on two consecutive tests.
• Male subjects must: a) Practice true abstinence or agree to use a condom during sexual contact with a pregnant female or a female of childbearing potential for 12 months after following CARCIKCD123/33 infusion and until CAR positive viable T cells are no longer present by quantitative polymerase chain reaction (qPCR) on two consecutive tests.
• Age limits: children (6 months - 17 years old) and adults (≥18 years old – 75 years old)
• Subjects with active AML/BPDCN with evidence of tumor involvement in the bone marrow and/or peripheral blood as determined by morphology and flow cytometric analysis and for whom no curative treatment option exists using currently available therapies.
• Availability of an at least haploidentical (i.e. 4/8 HLA matched by allele typing) familial or unrelated donor willing to and eligible for blood or lymphocyte donation for the CARCIKCD123/33 cell production
• Availability of a HCT donor, including a UCB, and patient eligible to a subsequent alloHCT
• Confirmed expression of CD123 and CD33 on leukemic blasts by flow cytometry.
• Prior treatment with anti-CD123– or anti-CD33–targeted therapy is permitted, provided a minimum washout period of 2 weeks has been completed before enrollment.
• Eastern Cooperative Oncology Group (ECOG) performance status equal to 2 or less for subject ≥ 16 years of age, ore Lansky >50 for subjects < 16 years of age
• Satisfactory organ functions