Mitochondrial Methylation in Type 2 Diabetes

Prior treatment

• Subjects receiving Gemfibrozil must not also be receiving a statin.

Other illness

• Subjects with a history of clinically significant heart disease (New York Heart Classification greater than grade II; more than non-specific ST-T wave changes on the EKG), peripheral vascular disease (history of claudication), or pulmonary disease (dyspnea on exertion of one flight or less; abnormal breath sounds on auscultation) will not be studied.
• Recent systemic or pulmonary embolus, untreated high-risk proliferative retinopathy, recent retinal hemorrhage, uncontrolled hypertension, systolic BP\>160, diastolic BP\>95, autonomic neuropathy, resting heart rate \>100, electrolyte abnormalities.

Lab values

• Subjects must not be receiving any of the following medications: thiazide or furosemide diuretics, beta-blockers, or other chronic medications with known adverse effects on glucose tolerance levels unless the patient has been on a stable dose of such agents for the past three months before entry into the study. Subjects must not be taking estrogens or other hormonal replacement therapy unless the subject has been on these agents on a stable dose for the prior three months. Subjects taking systemic glucocorticoids are excluded. Patients with type 2 diabetes will be excluded if they are taking thiazolidinediones.

• Subjects must be 21-55 years old
• Body Mass Index:
  • Lean, healthy BMI ≤25; Overweight,non-diabetic BMI 25-29.9; Obese, non-diabetic BMI 30-50; Type 2 Diabetes (per the American Diabetes Association criteria)
• Subjects must be able to communicate meaningfully with the investigator and must be legally competent to provide written informed consent.
• Female subjects must be non-lactating and will be eligible only if they have a negative pregnancy test throughout the study period, and must agree to use acceptable birth control (hormonal contraceptives, barrier methods, have an intrauterine device, or surgical sterilization)
• Subjects must have the following laboratory values:
  • Hematocrit ≥ 35 vol%
  • Serum creatinine ≤ 1.6 mg/dl
  • AST (SGOT) \< 2 times upper limit of normal
  • ALT (SGPT) \< 2 times upper limit of normal
  • Alkaline phosphatase \< 2 times upper limit of normal
  • Triglycerides \< 150 mg/dl for nondiabetics
  • Triglycerides \<300 for diabetics
  • INR ≤ 1.3
  • HbA1c ≤ 10