High-Intensity Interval Training and Regulatory T Cells

Prior treatment

• Previous chronic use of systemic glucocorticoids, immune-suppressors or immuno-modulators in the preceding 3 months.
• Participation in any other study that involved investigational drug or regimens in the preceding 12 months.
• Any immuno-related condition, chronic illness or prior treatment which, in the opinion of the investigator, precludes study participation.
• Recipient of blood transfusion in 3 months prior to blood drawing.
• Any symptomatic coronary artery disease prior enrollment within 3 months or any intervention for it in the past 6 months.

Other illness

• Patients with significant or active infection: HIV negative by ELISA or reverse transcription polymerase chain reaction (RT-PCR) [if ELISA is positive and RT-PCR is negative, the ELISA is considered false positive]; negative serology for Hepatitis B and C.
• Active malignancy or history of malignancy within 1 year.
• History of delayed or abnormal wound healing.
• Any orthopedic condition or chronic pain that prevents them from cycling.
• Any symptomatic current heart failure (New York Heart Association class III and IV), significant valvar heart disease.
• History of non-compliance.

Lab values

• Hemoglobin less than 9.0 g/dl at the time of blood drawing.
• Platelet count less than 100,000/* at the time of blood drawing.
• White blood count less than 4 and/or Absolute Neutrophil Count less than 1500/* at the time of blood drawing.

Age

• Age under 18 or over 65

Other

• BMI under 18.5 or between 25 and 30.
• Active lifestyle (more than 5,000 steps per day).
• Be pregnant or breastfeeding.
• Inability or unwillingness of participant to comply with study protocol or procedures.
• Impaired consent capacity.
• Any chronic use of anticoagulation.
• The study will not include any vulnerable population such as fetuses, pregnant women, children, or prisoners.

• Age between 18 and 65 years old.
• BMI between 18.5 and 25 or above 30. Rationale: we want to include "obesity" as categorical covariate in the influence of HIIT and discriminate the participants between "lean" (BMI range 18.5-25) and "obese" (BMI > 30)
• Sedentary lifestyle (fewer than 5,000 steps per day)
• Fluent in English, able to understand and provide informed consent.
• Subjects willing to participate in the study and comply with study requirements as evidenced by signed Institutional Review Board (IRB)-approved informed consent.