Acute Timed Exercise and 24h Metabolism

Prior treatment

• Previously diagnosed with type 2 diabetes
• Participation in another biomedical study within 1 month before the first study visit

Other illness

• Uncontrolled hypertension
• Hypomotility disorders of the gastro-intestinal tract
• Significant food allergies/intolerance (seriously hampering study meals)
• Any acute condition, exacerbation of chronic condition, or medical history that would in the investigator's opinion interfere with the study

Lab values

• Fasting plasma glucose
• ≥ 7.0 mmol/L
• Hemoglobin \< 7.8 mmol/L

Other

• Not meeting all inclusion criteria
• In case of an abnormal electrocardiogram at rest: this will be discussed with the responsible medical doctor
• Subjects who do not want to be informed about unexpected medical findings during the screening /study, or do not wish that their physician is informed
• Any contra-indication to the Equivital telemetric pill
• Extreme early bird or extreme night person (score ≤30 or ≥70 on morning-eveningness questionnaire self assessment)
• Heavily varying sleep-wake rhythm
• Night shift work during last 3 months
• Travel across \> 1 time zone in the last 3 months
• Frequent engagement in programmed exercise as judged by the investigator
• Using \> 400mg caffeine daily (more than 4 cups of coffee or energy drinks)
• Smoking
• Antecubital veins that present tremendous difficulty in obtaining frequent blood draws (e.g. too small)

• Signed informed consent
• Healthy (determined by dependent physician)
• Man or post-menopausal woman
• Age between 50 - 75 years
• BMI ≥ 25 kg/m2
• Pre-diabetes based on meeting at least one of the following criteria: impaired glucose tolerance defined as plasma glucose values ≥ 7.8 mmol/l and ≤ 11.1 mmol/l 120 minutes after glucose drink consumption during oral glucose tolerance test in screening, impaired fasting glucose defined as fasting plasma glucose ≥ 6.1 mmol/l and ≤ 6.9 mmol/l, insulin resistance defined as a glucose clearance rate ≤ 360 mL/kg/min as determined using OGIS120, HbA1c of 5.7-6.4%
• Regular sleeping habits (7 - 9h of daily sleep)
• Stable diet and weight: No weight gain or loss \> 3kg in the last three months