Microbiome and Atopy in Mali

Prior treatment

• Use of antibiotics in the 3 months prior to screening.
• Treatment with an investigational drug within 12 months prior to screening.
• Current smoker or tobacco use within 4 months prior to screening.

Other illness

• Current skin infections other than atopic dermatitis (e.g., scabies).
• Presence of current bacterial, viral, or fungal infection, with the exception of isolated onchomycosis, which is not exclusionary.
• Any other condition or factor that the investigator determines may significantly influence the results of testing.

Other

• Current pregnancy or lactation (determined by self-report).
• Active diarrhea as defined by three or more loose stools per day (Bristol stool scale score of 6 or 7).

• Aged ≥3 years.
• Able to provide informed consent (for ages ≥18 years) or has a parent or guardian who can provide informed consent on their behalf (for ages \<18 years).
• Positive Screening questionnaire for asthma or eczema.
• Diagnosed with atopic dermatitis or asthma via physician diagnosis and criteria as follows:
• Clinical diagnosis of atopic disease, as defined by Hanifin and Rajka criteria, that has been present for ≥3 months before the screening visit.
• Aged ≥3 years.
• Able to provide informed consent (for ages ≥18 years) or has a parent or guardian who can provide informed consent on their behalf (for ages \<18 years).
• Negative Screening questionnaire for asthma and eczema.
• In good general health as evidenced by medical history and not diagnosed with atopic dermatitis or asthma via physician diagnosis.
• Failure to meet Hanifin and Rajka criteria and \<3 on GINA guidelines for asthma diagnosis.
• No self-reported history of food allergy.