A Hypoxia-Inducible CAR-T Cell Targeting AXL and CD73 for Advanced Gastric Cancer

Prior treatment

• Patients with brain metastases who have active central nervous system symptoms. (Note: Patients with brain metastases who completed radiotherapy at least 3 months prior to enrollment and remain asymptomatic from CNS disease may be eligible).
• Receiving high-dose corticosteroids (\>10 mg/day of methylprednisolone or equivalent doses of other corticosteroids) or other immunosuppressive therapy within 14 days prior to enrollment.
• Intolerance or allergy to the investigational drug.
• Prior treatment with any form of adoptive T-cell therapy.
• Currently receiving anticoagulant or antiplatelet therapy.
• Major surgery or significant trauma within 4 weeks prior to enrollment.
• Judged by the investigator as unsuitable for cell therapy.

Other illness

• Active, known, or highly suspected autoimmune disease.
• Active, uncontrolled systemic infection.
• History of severe allergy to other monoclonal antibodies.
• History of interstitial lung disease.
• Evidence of organ failure:
  • Cardiac: Class III or IV heart failure (per NYHA or other applicable criteria).
  • Hepatic: Class C liver function as per Child-Pugh score.
  • Renal: Renal failure or uremia stage.
  • Pulmonary: Symptoms of severe respiratory failure.
  • Neurological: Impaired consciousness.
• Active Hepatitis B (HBsAg positive with detectable HBV DNA), active Hepatitis C (HCV RNA positive), or HIV antibody positive.
• History of organ transplantation.
• Active gastrointestinal bleeding, or history of gastrointestinal bleeding within the past month.
• History of drug abuse, or psychological/psychiatric disorders that may compromise compliance with the study.
• Any unstable condition or situation that may jeopardize the patient's safety or compliance in the study.
• History of other malignant tumors within the past 3 years.

Other

• Thyroid dysfunction.

• Aged 18 to 75 years, male or female.
• Clinically judged as unresectable Stage IV gastric cancer that has progressed on or is intolerant to standard therapy, or patients who voluntarily forego standard therapy.
• Patients must provide tumor tissue samples, with positive AXL and CD73 confirmed by immunohistochemical (IHC) staining at a central laboratory: AXL positivity rate ≥50% with staining intensity ≥++, and CD73 positivity rate ≥30% with staining intensity ≥+.
• ECOG Performance Status score of ≤ 1.
• Life expectancy of ≥ 3 months.
• At least one measurable lesion (≥ 1 cm).
• More than 4 weeks since the last failed treatment, and any toxicities from previous treatments must have recovered to Grade ≤ 1.
• Adequate organ function and bone marrow reserve, as defined by the following laboratory values within a specified period before enrollment:
  • 1\. Hemoglobin (Hb) ≥ 90 g/L. 2. Absolute Neutrophil Count (ANC) ≥ 1.0 × 10\^9/L. 3. Absolute Lymphocyte Count ≥ 0.5 × 10\^9/L. 4. Platelet count ≥ 100 × 10\^9/L. 5. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) ≤ 3 × upper limit of normal (ULN).
  • 6\. Serum amylase and lipase ≤ 1.0 × ULN. 7. Total bilirubin ≤ 1.5 × ULN. 8. Serum creatinine ≤ 1.5 × ULN or creatinine clearance ≥ 50 mL/min (calculated by the Cockcroft-Gault formula).
  • 9\. Prothrombin Time (PT) or International Normalized Ratio (INR), and Partial Thromboplastin Time (PTT) \< 1.5 × ULN. (Patients receiving warfarin or heparin anticoagulation therapy may be enrolled if no underlying abnormality in these parameters is suspected, but require close monitoring with at least weekly testing until INR is stable).
  • 9\. Adequate cardiac and pulmonary function. 10. Women of childbearing potential must have a negative pregnancy test within 7 days before treatment initiation. All subjects must agree to use effective contraception during the treatment period and for 1 year thereafter.
  • 11\. Voluntary signing of a written informed consent form, good compliance, and willingness to cooperate with follow-up.