SISTER: Sibling Oocyte Insemination With Frozen Sperm From Third Party Donors: Evaluation of Reproductive Techniques

MI-összefoglaló

A vizsgálat saját részvételi feltételeiből. Gép olvasta ki az oldalon álló regiszterszövegből, ezért tévedhet. Hogy ki vehet részt, azt a lenti regiszterszöveg és a vizsgálat csapata dönti el.

The trial is looking for women having IVF with frozen donor sperm at Shady Grove Fertility Center. Their eggs are split between two ways of joining egg and sperm: ICSI and conventional IVF.

Nem

Women only.

A betegség stádiuma

Infertility not caused by a male factor. Normal egg supply in the ovaries (AMH > 1 ng/mL, AFC > 10). More than 4 eggs collected at egg retrieval.

Biomarker vagy leletérték

AMH > 1 ng/mL and AFC > 10. Donor sperm after washing must have >50% motility and >5mil concentration.

A részvétel fő kizáró okai

• Donor sperm with significant problems, such as abnormal sperm count, movement or shape
• Any medical condition that rules out assisted reproduction (ART)

A regiszter feltételei, szó szerint

Mit akar kideríteni a vizsgálat

The goal of this clinical trial is to learn whether two different methods of helping eggs and sperm join-intracytoplasmic sperm injection (ICSI) and conventional in vitro fertilization (IVF)-lead to better embryo development when using frozen donor sperm in people who do not have male fertility problems. The main questions it aims to answer are:

A vizsgálat 2 csoportja

• Intracytoplasmic Sperm Injection (ICSI)A vizsgált kezelés

  In this arm, a portion of each participant's mature oocytes will be inseminated using intracytoplasmic sperm injection (ICSI). A single frozen-thawed donor sperm cell will be injected directly into each oocyte using micromanipulation techniques. The oocyte assignment to ICSI will be determined through randomized sibling-oocyte allocation. Embryos will be cultured under standard laboratory conditions and assessed for fertilization (2PN), blastulation, embryo quality, and early clinical pregnancy outcomes.

• Conventional Insemination (IVF)A vizsgált kezelés

  In this arm, a portion of each participant's oocytes will be inseminated using the conventional in vitro fertilization (IVF) method. Frozen-thawed donor sperm with normal post-thaw parameters will be co-incubated with oocytes in culture media for fertilization. The oocyte assignment to this method will be determined through randomized sibling-oocyte allocation. Embryos will be cultured under the same laboratory conditions and assessed for fertilization (2PN), blastulation, embryo quality, and early clinical pregnancy outcomes.